Friday, June 2, 2017

Letter to a Biology Student

Hi Biology Student,

     My name is Karthi Sankar, and I am 14 years old. I went to Foothill Elementary and Redwood Middle School. I was interested in computer programming and English at the beginning of the year. The classes I took were English, Geometry, Health/World Geography, Spanish 1, Introduction to Computer Programming, Physical Education, and of course, this class. My approach to school is to do the best that I can to the best of my ability. My interests outside of school include basketball. I played on the freshman basketball team this past year. I expected this class to be difficult, but something that I could still do well in. That is what it generally ended up being.
     Mr. Orre's expectations are that you come to class prepared and ready to learn and pay attention. You must be able to listen, stay on task during your working time, and be safe during labs. When you get to class on a normal day, you take out your notebook. In your notebook, you answer a question Mr. Orre writes on the board called a Do Now. What is your notebook for, you ask? It is like your own personal biology textbook that you will add to almost every day. You will understand this better as you read on. For homework, you do vodcast notes. This is usually your only homework. Vodcasts are lectures in the form of a video, where Mr. Orre explains a lesson/concept. They are anywhere from ten to twenty minutes long, usually somewhere in the middle. They usually take half an hour to an hour to complete, depending on how fast you take notes. You will fill out handouts with fill-in-the-blanks that go along with them. This is your homework every day. As you get better at taking notes, you will take notes without a handout on some vodcasts. For every unit, you will take notes on a corresponding chapter in the textbook. Anyway, back to a class day. After the Do Now, you review the vodcast you did for homework with your table group. Then, you do activities or labs to help reinforce what you learned for homework. The labs are pretty straight-forward, and you have to follow the procedure safely while giving yourself enough time to complete all of the steps. Your blog is a documentation of what you're doing all year. On it, you write reflections about each unit. This includes explaining what you have learned and talking about how you have grown during the course of the unit. You also include conclusions for labs and occasional posts as assignments. This is one of my favorite blog posts because I believe that it is my best reflection, also my last reflection. As the year went on, I got better at managing my time and organizing my work. The notebook really helped me keep everything together, as it was much better than a binder filled with papers. It is important for you to know that you have what it takes to do well in this class, but you need to keep on top of your work and do the best that you can on all of your assignments.
     Mr. Orre doesn't like people talking while he is talking. Listen while he talks, and then after the instructions, you can ask questions or discuss the assignment. He also doesn't take excuses for not finishing your work, unless they are legitimate. Complete your work well and on time. For assignments, you need to follow the directions and do the best that you can. You need to finish the whole assignment, not just parts of it. They must be thorough and informative. If you do not complete your homework regularly, you will not get a good grade in this class. Homework is a huge part of passing this class. If you do not do your assigned vodcast, you will be behind because you will not have learned what everyone else has, and therefore, you will not understand the discussion in class and possibly even the activity you do that day. To get good grades on tests, you will have to study. No question. If you don't look at your notebook from the time of the last vodcast to the test, you will probably not do well on said test. You need to find a way of studying that helps you prepare. Whether that's flashcards, quizzing yourself, doing the CFUs (short check for understanding quizzes you do after a vodcast), reading your Relate and Reviews (part of the vodcast), something completely different, or multiple methods, you should study before every test. To get the most out of this class, you need to give it your all, come to class prepared, and be ready to learn everyday. Now, I will describe three mistakes that I have made this year and hopefully you will learn from them. The first is not studying well or long enough for two tests. Because of this, I got two grades that I was not very proud of at the time. Do not put off studying to the last minute, or you could get the same result. Second, I procrastinated on a project and ended up not getting much data for it. I would have done better on the project if I did not do this. Again, don't let yourself get easily distracted or taken away from your work. Lastly, I didn't follow a part of a procedure for an experiment exactly right. My group still ended up getting results that we could use, but if I actually had made a large mistake, the data for our lab would have been messed up and not completely usable. The lesson from this is to follow the procedure during labs and be careful.
     Finally, I just want to rate the class for you. Using the grade scale, I would give it an A. I learned so much in this class, and I have become a better student because of it. I am able to complete work more efficiently and organize my work. As I said before, the notebook really helps with organization. The vodcasts helped me learn each concept very well, and you can also go back and re-watch them for another explanation. Mr. Orre is a great teacher, and you will learn a lot in his class and have fun too. Next year, I am taking Honors Chemistry. I decided to take this class because I was recommended for it, and I feel that I have enough skill in algebra, I can keep up with my work, and it seems interesting. Well, that's all I have to say. It's probably a lot to take at the moment, but don't worry. You'll get used to it. Have a great time in this class, and good luck!

Signed
- Karthi Sankar

   

Wednesday, May 31, 2017

Pig Dissection

The purpose of this dissection is to learn about the different structures inside a body by physically seeing them inside a fetal pig's body. We can also see how all of the systems are connected and situated inside the body. This dissection related to the vodcasts because we saw a physical digestive, circulatory, and respiratory system. The esophagus, stomach, and intestines are all connected. We also saw inside the heart by cutting it open. It was easier to imagine the flow of blood from the left atrium, to the left ventricle, to the right atrium, and to the right ventricle. We could also see the lungs of the pig. My favorite part of the dissection was making the video because we got to explain and find all the important parts of the pig. That way, we could better understand how its body works to allow it to live. This dissection was a valuable experience. We can see the systems in the body. It is a real-life view of what we were taught in the vodcasts. In addition, since the pig was unborn, it never had to be killed. It was good that each group had one pig because we could see inside clearly without lots of people crowding around one pig. We got to learn about the systems in the body and apply our knowledge by making the video. Below is a video my group created showing and explaining the important parts of the body.



Monday, May 22, 2017

Calabaza Creek Clean-up

On Sunday, May 20. I went to the Calabaza Creek in Cupertino. Then, for three hours, other volunteers and I cleaned up the creek. We used gloves and trash picker-uppers to pick up plastic and other pieces of trash at the river. We means groups because we had to work in pairs. Because there was not very much water, we were able to walk around the rocks and dirt to look for trash. The most common trash were cigarette butts. wrappers, and small pieces of paper or plastic. At the beginning, it was easier to find trash. After that, it took some time to look for each piece of plastic in the dirt and brush. We had to dig around and use good observational skills to find small pieces of plastic. These are the parts that fish can eat and get sick from. That is why it is important to find and pick up these as well as large boxes or bags. From this experience, I gained more knowledge about what trash is our rivers and how much of it there is. I learned how fish can get affected by eating plastic that we leave behind. I helped the community of Cupertino and other communities like it, and that is a good feeling to have. I gained experience on how to use my time. Since we had three hours, I had to plan out how to use my time and when to take breaks. I took away with me a sense that I had done something important and worthwhile. These kind of events can also raise awareness about the wasteful actions that people do all the time, such as littering, which can cause trash to get into rivers. I could do this again if I had the chance to. There is another clean-up in September, so it is possible that I could. One thing that I know now is that we take advantage of the nature around us. We need to protect it in order to continue to live and survive on this planet.




20 Time Individual Reflection

     My 20 time project was an experiment that was trying to find which food they preferred: genetically modified food or organic food. People rated the food on a scale of one to five on appearance and taste. The three foods we used were cheese, corn, and bananas. We also included some research that we did about the topic. I chose this project because at the time of choosing, we had learned about biotechnology and the modification of food. It seemed like an interesting topic to learn about and test. My partner and I did this project to inform people about the two different kinds of food. It was also done to see if there actually was a difference in preference among people. People can make their own decision about what they want to eat, but now they will have more information about each.
     Our initial plan was to do research for a few weeks and then conduct our experiment. Our first weeks were well-spent, and we looked at and got sources for our information. The original plan was to use about ten people as test subjects. That number went down to about seven. In the end, we ended up testing four people for each of the three foods. One thing that I would have done differently if we had more time would be to get more people to do the test. Then, we would have more generalized data at the end. Still, our experiment provides a small sample of what people prefer. Our experiment also could have happened earlier in the year. We were not getting the materials in time to conduct the experiment on the dates we set. This could have allowed us to involve more people. A success was that we finished the project. We explained the experiment, put together data tables, analyzed our results, and included some of the research we had done at the beginning. We put it all together in a document and included a works cited.
     If I did this experiment again, I would stick to our deadlines and get more test subjects. I got better at doing experiments because we had to put together our own instead of it being given to us. My researching also improved because we had to find reliable sources for our information. Since I worked with a partner, I made my communication skills better. We did not always agree on what was best for the experiment, but we compromised and ended up finishing. We were able to work together to complete work and reach a common goal. I learned that I could get things done (while not always meeting deadlines) when I put my mind to it. I need to procrastinate less if I want to get things done on time and done well. I am done with this challenge now because we completed our project. We collected our data and analyzed it. There is still more to learn about this topic, but this experiment can give people more information about what they eat. It's not too much information, but it will include things that people have never seen before.

Thursday, May 11, 2017

Unit 9 Reflection

     In this unit, we learned about the seven taxonomic levels and how life is classified into these levels. The seven levels are kingdom, phylum, class, order, family, genus, species. An addition is the three domains, Eukarya, Bacteria, and Archaea. Bacteria can be gram-positive or gram-negative, which means they either have less or more peptidoglycan in their cell walls. The different kinds of bacteria include cyanobacteria, symbiotic bacteria, and actinomycetes. Archaea live in extreme environments including anoxic mud, hydrothermal springs and digestive tracts. They challenge what we think is required for living. In the domain Eukarya, there are the kingdoms Fungi, Plantae, and Protista. Protista are very diverse and can be plant or animal-like. Fungi have cell walls made of chitin, absorb food through hyphae, and have fruiting bodies and spores, They include club and sac fungi and bread molds. Plantae (plants) have pollen, seeds, vascular systems, cuticles, and fruit. Bryophyta are seedless non-vascular mosses. Pterophyta are vascular seedless ferns. Gymnosperms have seeds in cones, and angiosperms have seeds in fruit. Throughout the different levels so far, there has been continual change between each kingdom. Each organism evolved to become a more recent one. This continues in the kingdom Animalia.

     In the kingdom Animalia, there are phyla of invertebrates and vertebrates. The first invertebrate phylum is Porifera, or the sessile filter-feeders, otherwise known as sponges. Another phylum is the Cnidarians, who are the first vertebrates to have specialized tissue. They include coral, hydras, and jellyfish. The next three phlya are closely related. Platyhelminthes are flatworms with an incomplete or no gut. The three classes are planarians, flukes, and tapeworms. Molluska have a digestive system, and they are snails, slugs, clams, and squids. Finally, Annelida are segmented worms with a coelom, like earthworms. A huge phylum is Arthopoda. The two classes in it are Insecta and Crustacea. The last phylum of invertebrates is Echinodermata, who have a water vascular system with a skeleton made of interlocking ossicles. The five classes include sea stars, sand dollars, basket stars, sea cucumbers, and sea lilies. Then comes the phylum of all vertebrates, Chordata. It starts with fish, specifically the jawless fish of class Agnatha. There are two other classes of fish. Condricthyes have a skeleton made of cartilage, and Osteicthyes have a bony skeleton. After the tranistional forms of fish climbed up onto land, the amiphibians (Amphibia) came into being. They include the salamanders, caecilians, and the frogs/toads. Reptilia is the class with the crocodilians, snakes, lizards, and turtles. They are ectotherms with a three-chambered heart and a cloaca. Aves (birds) evolved from therapod dinosaurs, and they have wings, feathers, and re-arranged hip muscles. The last and most recent class is the class Mammalia. Mammals are complex, social organisms with hair, a chewing jaw, and a four-chambered heart. The three gruops are marsupials, monotremes, and eutherians, or the most recent mammals. In each ensuing phylum and class, there is more diversity and more helpful traits that all lead to the existence of humans. As life went on, speciation and evolution occurred to create the organisms that exist today.
                                                                                                                                                                                      https://upload.wikimedia.org/wikipedia/commons/3/3b/AnimalsRelativeNumb ers.png 


     I wonder about what species we still have to discover and how we will classify them. There are so many species in the world that humans have not discovered yet. New phlya and classes could be created to accommodate a new, diverse species. Also, all of these organisms lived during different time periods, and we did a project showing a timeline of Earth's history. Click here to see my reflection on the project. In this unit, we also did presentations about different organisms to show exactly what on Earth evolved. My presentation went well and I was able to be in the time limit range. I believe that this is because I was well prepared, I practiced beforehand for my presentation, so I didn't read from my slides as much. One thing I could have improved on was putting less information onto the slides and not relying on it so much. By that, I mean that I could have spoke more from my own memory and not crowd the slides with information. I could have used it more as an aid than as the basis for my speaking. This gave me valuable experience with public speaking, as I will certainly have to do more of it in my life. I practiced speaking in front of an audience, and I got to see other people do it too. I learned a lot about other organisms on Earth, and each one was taught be a different person. Below is my presentation about the Australopithecus, the first ever upright-walking hominid.
    

Thursday, April 20, 2017

Geologic Timeline Reflection

     One major event is the mass extinction at the end of the Cretaceous period of the Mesozoic era. This event led to the rise of mammals, and that is why we are alive today. Without the dinosaurs ever going extinct, the dinosaurs might still be here on this planet. The history of the Earth in its later stages would be drastically changed and would not not be the same at all. Most of the mammals on Earth could be killed by dinosaurs. Another important event was when Pangaea formed in the Carboniferous period. If Pangaea never formed, the continents would not be like they are today. It's possible that the continents could have still been formed but they would not be the same. A third important event is the when Archaeopteris, the first successful trees, in the Devonian period came into being. If trees never existed, we would be be alive and the Earth would definitely be different and other organisms would be on Earth. Only organisms who don't need oxygen would survive. If any of these events never happened, the Earth would not be as it is today. 

     The longest era is the Precambrian, and it took up most of the time in the history of Earth. The Cenozoic era only just started and yet humans have had a large impact on the world. I was surprised when I found out that humans only take up a very short time in the span of everything since the Earth has formed. I thought that humans were on this planet for longer than they actually are. I think that I just didn't realized how long Earth has been in existence. I also did not really know about the Paleozoic era, and that is the era that I researched. Some important things happened in this era, like the diversification of vascular plants and the colonization of land by fungi, plants, and animals.

     Humans have had a large impact on the Earth. The good things that we have done have been to help ourselves to survive and reproduce on this planet. However, we have also had a bad impact on Earth. We are using its resources without knowing what it is doing to Earth. Also, some scientists have evidence that shows what we are doing, like using up the oil on Earth and releasing carbon dioxide in the atmosphere, is harming our planet. Some people are listening and are raising awareness, but not enough. A question that I have is, "Will we do anything to slow this mass extinction we are currently in?" Many people don't know about what is happening on the Earth now. There is climate change and ocean acidification and many other events happening. People are smart enough to come up solutions to the problems that we are facing, but enough minds are not on the problems. I do not have any more questions, but I wonder whether we as humans and as organisms on this planet will try to do something to help the planet, not only ourselves.


Monday, April 10, 2017

Unit 8 Reflection

This unit was called Constant Change, and it focused on evolution. It was about what causes evolution and what effect evolution has on populations. Natural selection is the driving force behind evolution, and populations, not individuals, evolve. Humans have come up with artificial selection. They decide what trait they want to see in an animal, mate two individuals with the desired trait, and see what they end up with. In other words, they are effectively breeding for a trait, and sometimes, the resulting individual will be different enough from its parents that it is considered a new breed. This selection is therefore not natural, and the end product is created because of the preference of a breeder. Evolution is defined as changes in allele frequency over time. Allele frequencies is the percentages of different alleles in populations. Natural selection is when traits that help organisms survive and reproduce continue into the populations that come after, while the unhelpful traits decrease in frequency and can eventually disappear. In other words, the population starts to look like the winners, while the losers' traits go away from the population.


Natural selection was demonstrated in the Bird Beak Lab and the Hunger Games Lab because different traits were tested for their ability to help organisms survive and reproduce. There is also evidence of evolution that has been found today. There is the usual, fossils, and they physically show the change in populations over time. Also, they can be dated to see how old they are. Embryology, the study of embryos and their development, and evo-devo, comparing the developmental processes of different organisms, are also new ways that scientists have found to prove common ancestry and evolution. Homologous structures, similar bones with different functions, and analogous structures, different bones with similar functions, show that individuals have a common ancestor. Different organisms having analogous structures has occurred because of convergent evolution. This happens when two unrelated organisms develop similar traits because they have to adapt to similar environments or ecological niches.


A geologic timeline can show how life on Earth has changed over time. Earth was formed about 4.6 billion years ago. The time since has been divided into eras, periods, and epochs. There have been five mass extinctions, and we are now in the midst of a sixth. The four eras are the Precambrian, Paleozoic, Mesozoic, and Cenozoic. Then, the eras are split into periods. Life appeared in the first era, then the Cambrian Explosion. After that, it was the Age of Reptiles, and finally, us humans came into being. Earth has changed rapidly throughout its history, and humans have just arrived onto the scene. We are still making a big impact on the Earth itself. New species occur through speciation, which is caused by reproductive isolation. It can be behavioral, temporal, or geographical. Populations evolve through three different kinds of selection. Disruptive favors the extreme phenotypes, stabilizing favors the intermediate, and directional favors either one of the extreme phenotypes. Evolution continues to happen today, and it is important to learn about it because this knowledge can help us to deal with change when it happens in our world.

 
 I want to learn more about the evidence that we have of evolution. It would be interesting to physically see fossils that show the changes over time, and see evolution that has taken place in past generations. This could be with analogous or homologous structures. I do not have any unanswered questions. I wonder about what will happen to our species. We have been on this planet for so little time, yet we have made so much change to this planet. I wonder if we will change what we are doing to try and help the planet instead of harming it. Everyone should become aware of what is going on in the world. I also wonder how our species will continue to evolve over time and if natural selection will play a role in our survival. Relating to assertiveness, I am mostly able to say what I want and speak about myself. I can probably become more clear with my priorities when I am doing something with other people. I can still continue to become more assertive while also listening to what others have to say, rather than dominating a conservation. That said, I should listen and contribute my own ideas that relate to what another person is saying. I can continue to become more confident in everything that I do and every situation.


Thursday, March 30, 2017

Hunger Games Lab

1) In this lab, we simulated natural selection by having different individuals with different traits compete to survive. The food was corks, and the variation was how we picked up the food. Stumpys, with the phenotype AA, picked up food between their wrists. Pinchers, with the phenotype aa, picked it up between their thumb and index finger. Knucklers, with the phenotype Aa, picked up food between the second knuckles of their index and middle fingers. An individual needed certain amounts of food to survive after each trial. Because of this, the populations kept changing.
2) The knucklers were the best at capturing food, and this is shown because their populations were mostly the highest. The individuals with this trait were able to pick up more food between their knuckles and could quickly collect and store the food.
3) The population did evolve because the allele frequencies of A and a kept changing throughout the lab. The frequency of the A allele changed from 0.50 at the first trial to 0.38 at the last. The a allele's frequency changed from 0.50 to 0.62. Evolution is changes in allele frequency over time, so evolution occurred.
4) The placement of the food was random and was an example of genetic drift. Depending on who was nearest to the food, they were able to collect the most food. Also, people cheating, not conforming to the trait that they were supposed to have, was random. Different people did this throughout the trials, and that affected the results. What was not random was the starting populations, when each trait had an equal population size. Also, the type of food and and the traits that individuals had weren't random.
5) If the food were bigger, it would probably be harder for knucklers to pick it up between their knuckles and the pinchers would have an advantage, and the stumpys could also. If the food were smaller, the stumpys could have a hard time picking it up, while the pinchers and knucklers could pick up more food at once.
6) If there was not incomplete dominance, then the intermediate species would have a different trait, and it would not be knucklers. This would affect allele frequency because these individuals would probably have more, or less, success than that of the knucklers.
7) Natural selection is the driving force behind evolution. Changes occur in a population naturally because of the enivroment or ecosystem an organism is in. Some species will have an advantage, while others will not. The frequency of the helpful trait will increase, while the other will decrease.
8) Some people tried to put much food as they could into their pockets and that way, they were able to store and collect more food than others. Also, people did cheat, and that helped them survive until the next trial. Some individuals probably would have died if they had completely followed the rules. Overall, these factors just helped more people survive, with the knucklers maintaining the highest population.
9) In evolution, populations, not individuals, evolve. When an organism is born, they are stuck with the genes their parents gave them. Only when they reproduce does the population evolve, because of a change in allele frequency. Natural selection acts on phenotypes, not genotypes. Depending on the traits an organism has, natural selection, based on whether the traits are helpful or not, either makes the population have that trait or decrease in individuals with that trait.
10) How would the addition of another trait affect the population's evolution?





Wednesday, March 8, 2017

Unit 7 Reflection

     This unit was about ecology and conservation biology. Ecology is the study of interactions between different organisms and their environment. Some big ideas of ecology are homeostasis and interdependence. The levels of organization of ecology are organisms, population, communities, ecosystems, biomes (an area of the world that contains many ecosystems), and the biosphere (Earth). We also learned about food chains and webs, and the differences between them. If one organism declines in an ecosystem, then, a domino effect is sent throughout the food web, and other populations could decrease. Next, we learned about the energy in ecosystems, which can be shown through biomass pyramids, pyramids of numbers, and energy pyramids. Only 10% of the energy at one trophic level (primary producers, primary consumers, secondary consumers, tertiary consumers, and quaternary consumers) is passed to the level, so there is less energy to go around as you go up the energy pyramid. That is why it is important to have many primary producers who transfer all of the energy to the next levels. Another part of ecology is population ecology, the study of populations in relation to the environment. Many different factors, like deaths or immigration, affect the rise and decrease of populations. Exponential growth is the doubling of a population over time. Logistic growth is when the rate of growth slows down as the carrying capacity is reached. A carrying capacity is the maximum point at which an environment support a population. The human population has been exponentially growing for some time now, and after more time, it is expected that it will reach its carrying capacity. At carrying capacity, the population will decline and rise again or level off (number of births will equal the amount of deaths).


     There are also different cycles that help to keep an ecosystem healthy. Ecological succession occurs with a sequence of community and ecosystem changes after a disturbance (ex. fire in Yellowstone causes the ecosystem to grow). Primary succession is when everything in a ecosystem has been harmed/disturbed, while secondary succession happens when part of an area is still intact. The water cycle includes evaporation and precipitation, while the carbon cycle involves cellular respiration. The nitrogen cycle can be the conversion of nitrogen gas into nitrates for plants or the breaking down of nitrogen from waste (into nitrates) from waste or dead organisms. Phosphorous makes up ATP, DNA, and lipids, and it is deposited by water and made available to plants. Decomposers and producers are critical to life. High biodiversity is important for ecosystems to be considered healthy because organisms are resistant to changes. Populations with producers & decomposers/tertiary & quaternery concumers are features of a healthy ecosystem. Four causes of species loss are climate change, overexploitation (harvesting organisms too fast/much so populations cannot rebound), introduced/exotic species, and habitat loss. There are five ways to conserve/protect ecosystems: identify and protect (biodiversity) hot spots, conservation (protect what we still have), smart planning (some solutions to help ecosystems (ex. movement corridors)), restoration (ex. jumpstart succession), and sustainable development (development that meets the needs of people today without limiting the ability of future generations). Finally, there are small things that each person can do to help, and if everyone does something small, there will be a big effect.


     I want to learn more about the different ways people are trying to conserve ecosystems through sustainable development or smart planning. The movement corridor and green roof are interesting ideas. I do not have any unanswered questions. I wonder about how people can raise awareness for the enviromental issues the earth is facing. Also, I wonder how we can all consume less and not run of resources. The Conservation Biologist project was a good project because I got to learn more about a place in the U.S. (the Great Plains) and the threats facing it. I thought my group worked well together, and everyone took part and did their work. Also, I got to practice explaining about a topic (in the video) besides just listening, and when you explain something to someone else, you learn it better yourself. I felt that our group could have recorded our video earlier because we went one day extra on our schedule to finish the presentation. Overall, our group did a good job on the project and created a educational video. My dominant conflict style is aggressiveness, with assertiveness the second, then passiveness, then finally passive-aggressiveness. I feel that in some situations, I could be aggressive or passive. I don't ask for help a lot, so that can improve my assertiveness. I don't think I am passive-aggressive often, but sometimes I can be. I think that whether I am aggressive or passive depends on where I am or who I am with, but I should try to be assertive most of the time. I could voice my ideas more, think about what I say before I say it, and say what I think is the right thing to do.


Wednesday, February 1, 2017

Unit 6 Reflection

     This unit focused mostly on biotechnology and the ethics attached to it. Biotechnology itself is the study or manipulation of living things in order to benefit mankind. Examples of it include biofuels, CSI work, and fermentation. Also, genetically modified organisms, or GMOs, are created using biotech. Ethics are also discussed when talking about different biotechnological possibilites, and the specific name for that is bioethics. Bioethics is the study of decision-making as it applies to moral decisions that have to be made because of advances in biology, medicine, or technology. A few examples of bioethical questions are: Should humeans be cloned to be organ donors?; Should drug companies be allowed to patent genes? and Should we use stem cells from embryos in order to treat diseases of living people? We also learned about recombinant DNA. Recombinant DNA is DNA that has been made by inserting the DNA of one organism into the DNA of another. It is created using restriction enzymes and ligase. The enzymes look for a specific sequence in DNA. From there, a new DNA strand can be formed by attaching a new strand to the sticky ends of the old one, using the enzyme called ligase. This DNA can also be used in bacteria. When plasmids and recombinant DNA are mixed and put in bacteria, the bacteria can grow a specific protein which can be extracted and used. Finally, there was a vodcast about the pGLO lab, which included a review of gel electrophoresis. It involves a plasmid called pGLO, ampicillin, and arabinose. It will be explained more later in this reflection.


https://upload.wikimedia.org/wikipedia/commons/d/de/Biotech-firm-application.png

     My strengths were almost the same they always are. I did all the vodcasts and other assignments and turned them on time. I paid attention in class and during labs, while also doing parts of the lab. I also believe that I have my topic for my 20 time project. A weakness I have is probably procrastinating because sometimes I do the vodcasts the day before they are due. Also, I left the textbooks until near the due date. I also think that I can have better lab skills because during the pGLO lab, I reused a pipette when the procedure said to get a new one after each time you use it.

   Successful transformation of E. coli with pGLO plasmid! #biology #gofalconpower pic.twitter.com/a0lgGzVul2
     The 3 labs we did were the electrophoresis virtual lab, the candy electrophoresis lab, and the pGLO lab. The virtual lab was going through the steps of electrophoresis and using it on DNA (all virtually). First, you extract DNA and then load it into a gel. At one end is a negative charge and at the other end is a positive charge. DNA will move towards the positive charge because it is negatively charged. If a strand is long, it will move slower and therefore, faster, and smaller strands will move quicker therefore longer. Then, you can measure the length of the DNA in bp (base pairs). The candy electrophoresis lab was actually electrophoresing different dyes of different candies to see how long each of their DNA strands are. The pGLO lab was using bacterial transformation. Using a plasmid called pGLO (some with DNA and some with no DNA), we had to combine the plasmid with arabinose, ampicillin, and GFP (glowing fluorescent protein). Then, we had to freeze and heat shock the bacteria so that it would pick up the plasmid. On three different plates, we tried to grow the bacteria. If it picked up the plasmid, the bacteria grew. Adding arabinose caused one of the bacteria to glow (an expected result). As always, I got better at doing the labs and following the procedure while also reinforcing the concepts that I had learned in the vodcasts, like gel electrophoresis and bacterial transformation.

https://upload.wikimedia.org/wikipedia/commons/thumb/6/60/Gel_electrophoresis_2.jpg/320px-Gel_electrophoresis_2.jpg 

      I have no questions that I want to have answered. I want to learn more about genetically modified organisms. Also, bioethics is interesting to discuss because there can be so many different views on one topic or question, and it is always important to hear everyone's ideas to make an informed decision for yourself. My SMART goals were to study in different ways for tests and participate more in class. I think that I have partly acheived my second goal because I have talked more in small dicussion groups, like the Biotech World Cafe. I have not made progress on the first goal. So, my next steps are to raise my hand more during class and make some changes to my studying technique.

Monday, January 30, 2017

pGLO Lab

pGLO Observations , Data Recording & Analysis
1.
Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below.
Plate
Number of Colonies
Color of colonies under room light
Color of colonies under   UV light
- pGLO LB
0Light GrayDark Gray
- pGLO LB/amp
NoneNoneNone
+ pGLO LB/amp
6Light GrayDark Gray
+ pGLO LB/amp/ara
7WhiteGreen


2.
What two new traits do your transformed bacteria have?
The transformed bacteria now glows (because of the GFP) and is resistant to ampicilin.



3.
Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.

There are over a million bacteria in one colony. I predict that about ten colonies are in a hundred microliters (uL). Therefore, there would be about ten or eleven million bacteria in one hundred microliters.

4.
What is the role of arabinose in the plates?
The arabinose provides a way to control the expression of the glowing fluorescent protein (GFP) gene. If it is present, the GFP will cause to the bacteria to glow. If it isn't, then that won't happen.



5.
List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
- Fluorescence microscopy
GFP is used with fluorescence microscopes, microscopes that use fluorescence to study properties of substances. GFP has advanced and redefined this field and will cause some substances studied to fluoresce.
- Macro-photography
Certain biological processes, like the spread of virus infections, can be followed using labeling. This labeling is done with GFP. Epifluourescent camera attachments are now used instead of UV light.
- Transgenic Animals
Some animals have been genetically engineered to glow using GFP. These animals can help scientists study certain things, like human diseases, and were also marketed as pets.


6.
Give an example of another application of genetic engineering.
Bacteria can modified to make certain proteins that can obtained and used. For
example, insulin and spider silk, which are difficult to get naturally, can be made
by genetically engineered bacteria.

















Thursday, January 19, 2017

Candy Electrophoresis Lab

In this lab we electrophoresed four reference dyes and four candies: red Mike and Ike's, purple Skittles, green M&Ms, and orange Reeses. The blue reference dye didn't match any of the candies' or references' length. Every dye had only one color band. Also, none of the dyes moved towards the cathode. The purple and red candy had a different dye color. The red and purple areas were also larger than those of the reference dyes. These dyes are probably just variations of the red reference dye. The dyes Citrus red 2 and Fast green FCF would migrate similarly to the dyes in this lab because they have similar structures to the reference dyes.

             
              

Dog food manufacturers may put artifical food colors in dog food to make it look more appealing to buy. Also, people will buy the food if it looks good and has color. Additionally, it could taste good for dogs. Foods I eat that would probably have artificial dyes are chips, candy (like M&Ms), macaroni and cheese, and soda (not too often). Artificial food colors can be preferable to companies rather than natural food dyes because it could be less expensive, taste better, and look appealing to eat. The length of the DNA fragment and the molecules in the dye control what distance the dye migrates. Electricity is the force that helps to move the dyes through the gel. Positive and negative charges at each end of the gel causes the molecules to separate by size. The smaller the DNA fragment, the longer/faster the fragment goes toward the positive charge (because DNA has a negative charge). The opposite happens for large fragments. DNA molecules of the lengths 600, 1000, 2000, 5000 daltons would separate like this: The 5000 wouldn't go far and would be closest to the cathode. The 600 would go the farthest distance. The 1000 would be second and the 2000 would be third, closer to the 5000-length molecule.


Tuesday, January 10, 2017

SMART Goals

     One goal that I have for this semester is to study better for tests. I will find a way to study that helps me remember the material I learn from each unit. I can try different studying methods, such as using Quizlet, answering questions from Relate and Reviews and Do Nows, taking the CFUs again, and re-watching parts of vodcasts. For each unit, I can try each or multiple techniques. By the end of the semester, I hope to study in a way that helps and benefits me, so that I can use this method or one like it for another class.
     Another goal that I will try to accomplish is participating more in classes. Participation is important, and it helps you to speak better and for longer amounts of time. I can start by raising my hand a few times. As the semester goes on, I want to share my answers or ideas more often, but not all the time. Also, I can be more involved when doing things in class like projects or labs. This could help me overall have a better experience in class and gain more knowledge.