pGLO Observations , Data Recording & Analysis
1.
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Obtain your team plates. Observe your set of “+pGLO” plates under room light and with UV light. Record numbers of colonies and color of colonies. Fill in the table below.
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2.
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What two new traits do your transformed bacteria have?
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The transformed bacteria now glows (because of the GFP) and is resistant to ampicilin.
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3.
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Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
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There are over a million bacteria in one colony. I predict that about ten colonies are in a hundred microliters (uL). Therefore, there would be about ten or eleven million bacteria in one hundred microliters.
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4.
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What is the role of arabinose in the plates?
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The arabinose provides a way to control the expression of the glowing fluorescent protein (GFP) gene. If it is present, the GFP will cause to the bacteria to glow. If it isn't, then that won't happen.
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5.
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List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
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- Fluorescence microscopy
GFP is used with fluorescence microscopes, microscopes that use fluorescence to study properties of substances. GFP has advanced and redefined this field and will cause some substances studied to fluoresce. - Macro-photography Certain biological processes, like the spread of virus infections, can be followed using labeling. This labeling is done with GFP. Epifluourescent camera attachments are now used instead of UV light. - Transgenic Animals Some animals have been genetically engineered to glow using GFP. These animals can help scientists study certain things, like human diseases, and were also marketed as pets. | ||
6.
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Give an example of another application of genetic engineering.
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Bacteria can modified to make certain proteins that can obtained and used. For
example, insulin and spider silk, which are difficult to get naturally, can be made
by genetically engineered bacteria.
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