Human DNA Extraction Lab

     In this lab, we asked the question, "Can DNA be separated from cheek cells, and if so at what point do you predict you will be able to see the DNA?". We found that you could, if you follow the process correctly, after a process called lysis and during precipitation. I was not able to extract DNA possibly because of an error during the experiment, but others did extract their own DNA. The DNA came out as a precipitate into a layer of cold alcohol. This occurred because the alcohol is nonpolar and the DNA is polar. This data supports my claim because this occurs after lysis, during precipitation. Lysis is the disintegration of a cell by rupture of the cell membrane.
     Our data contradicts the expected results because DNA was not extracted. This could have been because while the isopropanol alcohol was poured into the solution too quickly and not from the right angle. Also, during the stage of homogenization (preparation of a suspension of cell constituents from tissue by physical treatment by a liquid, the liquid being Gatorade), too much Gatorade or detergent (used for lysis) could have been measured in too large or small quantities, which could have changed the process or made it not work. One recommendation that I have is to measure quantities carefully and accurately. Another is that all actions done in the lab, like pouring alcohol, should be done in the proper way.
     This lab was done to demonstrate how DNA is extracted and when you extract it. From this lab, I learned about homogenization, lysis, and precipitation, which helps me understand the concept of DNA extraction and the structure of DNA. Based on my experience in this lab, if my or someone else's DNA is needed in a situation, I understand how to extract it from human cells.